版权说明 帮助中心
首页 > 成果 > 详情

Assessment of candidate plant DNA barcodes using the Rutaceae family

SCI-EMedline
WOS被引频次:32
认领
导出
Link by DOI
反馈
分享
QQ微信 微博
成果类型:
期刊论文
作者:
Luo Kun;Chen ShiLin*;Chen KeLi;Song JingYuan;Yao Hui;Ma XinYe;Zhu YingJie;Pang XiaoHui;Yu Hua;Li XiWen;Liu Zhen
通讯作者:
Chen ShiLin
作者机构:
[Pang XiaoHui; Luo Kun; Ma XinYe; Li XiWen; Chen ShiLin; Song JingYuan; Yao Hui; Yu Hua] Chinese Acad Med Sci, Peking Union Med Coll, Inst Med Plant Dev, Beijing 100193, Peoples R China.
[Liu Zhen; Luo Kun; Chen KeLi] Hubei Univ Chinese Med, Coll Pharm, Wuhan 430061, Peoples R China.
[Zhu YingJie] SW Jiaotong Univ, Sch Biosci & Engn, Chengdu 610031, Peoples R China.
[Li XiWen] Tsinghua Univ, Dept Chem, Beijing 100084, Peoples R China.
通讯机构:
[Chen ShiLin] Chinese Acad Med Sci, Peking Union Med Coll, Inst Med Plant Dev, Beijing 100193, Peoples R China.
语种:
英文
关键词:
DNA barcoding;ITS2;Rutaceae;identification
期刊:
Science China. Life sciences
ISSN:
1674-7305
年:
2010
卷:
53
期:
6
页码:
701-708
文献类别:
WOS:Article
所属学科:
ESI学科类别:生物学与生物化学;WOS学科类别:Biology
入藏号:
基金类别:
National International Cooperation Program [2007DFA30990]; Special Scientific Research Project of Health Profession [200802043]
机构署名:
本校为其他机构
院系归属:
生命科学与工程学院
摘要:
DNA barcoding is a rapidly developing frontier technology that is gaining worldwide attention. Here, seven regions (psbA-trnH, matK, ycf5, rpoC1, rbcL, ITS2, and ITS) with potential for use as DNA barcodes were tested for their ability to identify 300 samples of 192 species from 72 genera of the family Rutaceae. To evaluate each barcode's utility for species authentication, PCR amplification efficiency, genetic divergence, and barcoding gaps were assessed. We found that the ITS2 region exhibited the highest inter-specific divergence, and that this was significantly higher than the intra-specific variation in the "DNA barcoding gap" assessment and Wilcoxon two-sample tests. The ITS2 locus had the highest identification efficiency among all tested regions. In a previous study, we found that ITS2 was able to discriminate a wide range of plant taxa, and here we confirmed that ITS2 was also able to discriminate a number of closely related species. Therefore, we propose that ITS2 is a promising candidate barcode for plant species identification.
参考文献:
Hebert P D, Cywinska A, Ball S L, Biological identifications through DNA barcodes. Proc R Soc Biol Sci SerB, 2003, 270: 313–321 10.1098/rspb.2002.2218, 1:CAS:528:DC%2BD3sXktVWiu7g%3D
Gregory T R. DNA barcoding does not compete with taxonomy. Nature, 2005, 434: 1067 10.1038/4341067b, 15858548, 1:CAS:528:DC%2BD2MXjsF2ltrk%3D
Miller S E. DNA barcoding and the renaissance of taxonomy. Proc Natl Acad Sci USA, 2007, 104: 4775–4776 10.1073/pnas.0700466104, 17363473, 1:CAS:528:DC%2BD2sXjvFCjsLo%3D
Hebert P D, Ratnasingham S, deWaard J R. Barcoding animal life: cytochrome c oxidase subunit 1 divergences among closely related species. Proc R Soc Biol Sci SerB, 2003, 270: S96–S99 10.1098/rsbl.2003.0025, 1:CAS:528:DC%2BD3sXns1Smsbo%3D
Vences M, Thomas M, Bonett R M, Deciphering amphibian diversity through DNA barcoding: chances and challenges. Philos Trans R Soc Lond B Biol Sci, 2005, 360: 1859–1868 10.1098/rstb.2005.1717, 16221604, 1:CAS:528:DC%2BD2MXhtlSjsrjE

反馈

验证码:
看不清楚,换一个
确定
取消

成果认领

标题:
用户 作者 通讯作者
请选择
请选择
确定
取消

提示

该栏目需要登录且有访问权限才可以访问

如果您有访问权限,请直接 登录访问

如果您没有访问权限,请联系管理员申请开通

管理员联系邮箱:yun@hnwdkj.com