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Use of ITS2 region as the universal DNA barcode for plants and animals.

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WOS被引频次:223
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成果类型:
期刊论文
作者:
Yao, Hui;Song, Jingyuan;Liu, Chang;Luo, Kun;Han, Jianping;Li, Ying;Pang, Xiaohui;Xu, Hongxi;Zhu, Yingjie;Xiao, Peigen;Chen, Shilin
通讯作者:
Yao, H
作者机构:
[Xu, Hongxi] Hong Kong Jockey Club Inst Chinese Med, Chinese Med Lab, Hong Kong, Hong Kong, Peoples R China.
[Chen, Shilin; Liu, Chang; Han, Jianping; Yao, Hui; Pang, Xiaohui; Song, Jingyuan; Li, Ying; Xiao, Peigen; Luo, Kun] Chinese Acad Med Sci, Peking Union Med Coll, Inst Med Plant Dev, Beijing 100037, Peoples R China.
[Zhu, Yingjie] SW Jiaotong Univ, Sch Biosci & Engn, Chengdu, Sichuan, Peoples R China.
[Luo, Kun] Hubei Univ Chinese Med, Coll Pharm, Wuhan, Hubei, Peoples R China.
通讯机构:
[Yao, H] Chinese Acad Med Sci, Peking Union Med Coll, Inst Med Plant Dev, Beijing 100037, Peoples R China.
语种:
英文
期刊:
PLOS ONE
ISSN:
1932-6203
年:
2010
卷:
5
期:
10
文献类别:
WOS:Article
所属学科:
ESI学科类别:植物学&动物科学;WOS学科类别:Multidisciplinary Sciences
入藏号:
WOS:000282359300009;PMID:20957043
基金类别:
National Natural Science Foundation of China [30970307]; National Key Technology R&D Program in the 11th Five-Year Plan of China [2007BAI27B01]
机构署名:
本校为其他机构
院系归属:
生命科学与工程学院
摘要:
BACKGROUND: The internal transcribed spacer 2 (ITS2) region of nuclear ribosomal DNA is regarded as one of the candidate DNA barcodes because it possesses a number of valuable characteristics, such as the availability of conserved regions for designing universal primers, the ease of its amplification, and sufficient variability to distinguish even closely related species. However, a general analysis of its ability to discriminate species in a comprehensive sample set is lacking. METHODOLOGY/PRINCIPAL FINDINGS: In the current study, 50,790 plant and 12,221 animal ITS2 sequences downloaded from GenBank were evaluated according to sequence length, GC content, intra- and inter-specific divergence, and efficiency of identification. The results show that the inter-specific divergence of congeneric species in plants and animals was greater than its corresponding intra-specific variations. The success rates for using the ITS2 region to identify dicotyledons, monocotyledons, gymnosperms, ferns, mosses, and animals were 76.1%, 74.2%, 67.1%, 88.1%, 77.4%, and 91.7% at the species level, respectively. The ITS2 region unveiled a different ability to identify closely related species within different families and genera. The secondary structure of the ITS2 region could provide useful information for species identification and could be considered as a molecular morphological characteristic. CONCLUSIONS/SIGNIFICANCE: As one of the most popular phylogenetic markers for eukaryota, we propose that the ITS2 locus should be used as a universal DNA barcode for identifying plant species and as a complementary locus for CO1 to identify animal species. We have also developed a web application to facilitate ITS2-based cross-kingdom species identification (http://its2-plantidit.dnsalias.org).
参考文献:
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