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Immobilization of selenocystamine on TiO2 surfaces for in situ catalytic generation of nitric oxide and potential application in intravascular stents

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WOS被引频次:73
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成果类型:
期刊论文
作者:
Weng, Yajun;Song, Qiang;Zhou, Yujuan;Zhang, Liping;Wang, Jin;Chen, Junying;Leng, Yongxiang;Li, Suiyan;Huang, Nan*
通讯作者:
Huang, Nan
作者机构:
[Weng, Yajun; Zhou, Yujuan; Leng, Yongxiang; Huang, Nan; Wang, Jin; Chen, Junying; Song, Qiang; Zhang, Liping] SW Jiaotong Univ, Sch Mat Sci & Engn, Chengdu, Peoples R China.
[Li, Suiyan] SW Jiaotong Univ, Sch Life Sci & Engn, Chengdu, Peoples R China.
通讯机构:
[Huang, Nan] SW Jiaotong Univ, Sch Mat Sci & Engn, Chengdu, Peoples R China.
语种:
英文
关键词:
Surface immobilization;Selenocystamine;Dopamine;Nitric oxide;Catalytic activity
期刊:
Biomaterials
ISSN:
0142-9612
年:
2011
卷:
32
期:
5
页码:
1253-1263
文献类别:
WOS:Article;EI:Journal article (JA)
所属学科:
ESI学科类别:材料科学;WOS学科类别:Engineering, Biomedical;Materials Science, Biomaterials
入藏号:
WOS:000287073000001;EI:20104913467100;PMID:21093045
基金类别:
Key Basic Research Project [2005CB623904, 2011CB606204]; National Natural Science Foundation of China [30900295, 30831160509]; China Postdoctoral Science Foundation [20090450171, 201003704]
机构署名:
本校为第一且通讯机构
院系归属:
材料科学与工程学院
生命科学与工程学院
摘要:
Immobilization of selenocystamine on TiO2 film deposited on silicon wafer and 316 stainless steel stents for catalytic generation of nitric oxide was described. Polydopamine was used as the linker for immobilization of selenocystamine to the TiO2 surface. In vitro stability of the immobilized selenocystamine was investigated and the result shows surface selenium loss occurs mostly in the first four weeks. The selenocystamine immobilized surface possesses glutathione peroxidase (GPx) activity, and the activity increases with the amount of grafted polydopamine. Such selenocystamine immobilized surfaces show the ability of catalytically decomposing endogenous S-nitrosothiols (RSNO), generating NO; thus the surface displays the ability to inhibit collagen-induced platelet acitivation and aggregation. Additionally, smooth muscle cells are inhibited from adhering to the selenocystamine immobilized sample when RSNO is added to the culture media. ELISA analysis reveals that cGMP in both platelets and smooth muscle cells significantly increases with NO release on selenocystamine immobilized samples. Two months in vivo results show that selenocystamine immobilized stents are endothelialized, and show significant anti-proliferation properties, indicating that this is a favorable method for potential application in vascular stents. (C) 2010 Published by Elsevier Ltd.
参考文献:
Abuo-Rahma GEAA, 2005, EUR J MED CHEM, V40, P281, DOI 10.1016/j.ejmech.2004.11.003
Aoki J, 2005, J AM COLL CARDIOL, V45, p69A
Cha W, 2007, BIOMATERIALS, V28, P19, DOI 10.1016/j.biomaterials.2006.08.019
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CHAUDIERE J, 1992, ARCH BIOCHEM BIOPHYS, V296, P328, DOI 10.1016/0003-9861(92)90580-P

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