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Cellulase activities in biomass conversion: measurement methods and comparison

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WOS被引频次:98
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成果类型:
期刊论文
作者:
Dashtban, Mehdi;Maki, Miranda;Leung, Kam Tin;Mao, Canquan;Qin, Wensheng*
通讯作者:
Qin, Wensheng
作者机构:
[Mao, Canquan] SW Jiaotong Univ, Sch Life Sci & Engn, Chengdu, Peoples R China.
[Maki, Miranda; Dashtban, Mehdi; Qin, Wensheng] Lakehead Univ, Biorefining Res Initiat, Thunder Bay, ON P7B 5E1, Canada.
[Maki, Miranda; Dashtban, Mehdi; Qin, Wensheng; Leung, Kam Tin] Lakehead Univ, Dept Biol, Thunder Bay, ON P7B 5E1, Canada.
[Qin, Wensheng] Lakehead Univ, Biorefining Res Initiat, 955 Oliver Rd, Thunder Bay, ON P7B 5E1, Canada.
通讯机构:
[Qin, Wensheng] Lakehead Univ, Biorefining Res Initiat, 955 Oliver Rd, Thunder Bay, ON P7B 5E1, Canada.
语种:
英文
关键词:
Biofuel;biomass;bioconversion;cellulase;cellulase assays
期刊:
Critical Reviews in Biotechnology
ISSN:
0738-8551
年:
2010
卷:
30
期:
4
页码:
302-309
文献类别:
WOS:Review;EI:Journal article (JA)
所属学科:
ESI学科类别:生物学与生物化学;WOS学科类别:Biotechnology & Applied Microbiology
入藏号:
WOS:000284317300004;EI:20104713413186
基金类别:
Ontario Graduate Scholarship; NSERC; NSERC-RCD; NOHFC; Ontario Research Chair
机构署名:
本校为其他机构
院系归属:
生命科学与工程学院
摘要:
Cellulose, the major constituent of all plant materials and the most abundant organic molecule on the Earth, is a linear biopolymer of glucose molecules, connected by β-1,4-glycosidic bonds. Enzymatic hydrolysis of cellulose requires mixtures of hydrolytic enzymes including endoglucanases, exoglucanases (cellobiohydrolases), and β-glucosidases acting in a synergistic manner. In biopolymer hydrolysis studies, enzyme assay is an indispensable part. The most commonly used assays for the individual enzymes as well as total cellulase activity measurements, including their advantages and limitations, are summarized in this review article. In addition, some novel approaches recently used for enzyme assays are summarized.
参考文献:
Baronas R., 2003, Nonlinear Analysis Modelling and Control, V8, P3
Bhatia Y, 2005, APPL MICROBIOL BIOT, V66, P527, DOI 10.1007/s00253-004-1754-8
BREUIL C, 1986, BIOTECHNOL BIOENG, V28, P1653, DOI 10.1002/bit.260281109
Caspi J, 2008, J BIOTECHNOL, V135, P351, DOI 10.1016/j.jbiotec.2008.05.003
Chandra M, 2009, J IND MICROBIOL BIOT, V36, P605, DOI 10.1007/s10295-009-0544-9

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