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Electrospun fibrous mats with high porosity as potential scaffolds for skin tissue engineering

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WOS被引频次:179
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成果类型:
期刊论文
作者:
Zhu, Xinli;Cui, Wenguo;Li, Xiaohong;Jin, Yan
通讯作者:
Li, XH
作者机构:
[Li, Xiaohong; Jin, Yan; Cui, Wenguo; Zhu, Xinli] Key Laboratory of Advanced Technologies of Materials, Ministry of Education, Southwest Jiaotong University, Chengdu 610031, China
[Zhu, Xinli] Research and Development Center for Tissue Engineering, College of Stomatology, Fourth Military Medical University, Xi'an 710032, China
通讯机构:
[Li, XH] SW Jiaotong Univ, Sch Mat Sci & Engn, Minist Educ, Key Lab Adv Technol Mat, Chengdu 610031, Peoples R China.
语种:
英文
关键词:
Average pore size - Cell infiltration - Electrospinning methods - Electrospun - Electrospun fibrous mats - Extra cellular matrices - High porosity - Human Dermal Fibroblasts - Immunohistochemistry - Mass transport - Mechanical supports - Micro-pores - Porous scaffolds - Rotating frames - Skin tissue engineering - Toluidine blue - Unifor m distribution
期刊:
Biomacromolecules
ISSN:
1525-7797
年:
2008
卷:
9
期:
7
页码:
1795-1801
文献类别:
WOS:Article;EI:Journal article (JA)
所属学科:
ESI学科类别:生物学与生物化学;WOS学科类别:Biochemistry & Molecular Biology;Chemistry, Organic;Polymer Science
入藏号:
WOS:000257639500012;EI:20083211442843;PMID:18578495
机构署名:
本校为第一且通讯机构
院系归属:
材料科学与工程学院
摘要:
Diffusional limitations of mass transport have adverse effects on engineering tissues that normally have high vascularity and cellularity. The current electrospinning method is not always successful to create micropores to encourage cell infiltration within the scaffold, especially when relatively large-sized pores are required. In this study, a slow rotating frame cylinder was developed as the collector to extend the pore size and increase the porosity of electrospun fibrous scaffolds. Fibrous mats with porosity as high as 92.4% and average pore size of 132.7 μm were obtained. Human dermal fibroblasts (HDFs) were seeded onto these mats, which were fixed on a cell-culture ring to prevent the shrinkage and contraction during the incubation. The viability test indicated that significantly more HDFs were generated on highly porous fibrous mats. Toluidine blue staining showed that the highly porous scaffold provided mechanical support for cells to maintain uniform distribution. The cross-section observations indicated that cells migrated and infiltrated more than 100 μm inside highly porous fibrous mats after 5 d incubation. The immunohistochemistry analysis demonstrated that cells began secreting collagen, which is the main constituent of extracellular matrix. It is supposed that highly porous electrospun fibrous scaffolds could be constructed by this elaboration and may be used for skin tissue engineering. ©2008 American Chemical Society.
参考文献:
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