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Electrospun fibrous scaffolds with continuous gradations in mineral contents and biological cues for manipulating cellular behaviors

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WOS被引频次:45
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成果类型:
期刊论文
作者:
Bin Zou;Yaowen Liu;Xiaomin Luo;Fang Chen;Xueqin Guo;Xiaohong Li
通讯作者:
Li, Xiaohong
作者机构:
[Xiaohong Li; Bin Zou; Xueqin Guo; Yaowen Liu; Fang Chen; Xiaomin Luo] SW Jiaotong Univ, Sch Mat Sci & Engn, Minist Educ, Key Lab Adv Technol Mat, Chengdu 610031, Peoples R China.
[Xiaohong Li; Bin Zou] SW Jiaotong Univ, Sch Life Sci & Engn, Chengdu 610031, Peoples R China.
通讯机构:
[Li, Xiaohong] SW Jiaotong Univ, Sch Mat Sci & Engn, Minist Educ, Key Lab Adv Technol Mat, Chengdu 610031, Peoples R China.
语种:
英文
关键词:
Functionally graded tissues;Hydroxyapatite gradient;Electrospun fibrous mats;Cellular behaviors;Gene expression
期刊:
Acta Biomaterialia
ISSN:
1742-7061
年:
2012
卷:
8
期:
4
页码:
1576-1585
文献类别:
WOS:Article
所属学科:
ESI学科类别:材料科学;WOS学科类别:Engineering, Biomedical;Materials Science, Biomaterials
入藏号:
基金类别:
National Natural Science Foundation of China [20774075, 51073130]; National Basic Research Program of China (973 Program) [2012CB933602]; Fundamental Research Funds for the Central Universities [SWJTU11CX126, SWJTU11ZT10, SWJTU09ZT21]
机构署名:
本校为第一且通讯机构
院系归属:
材料科学与工程学院
生命科学与工程学院
摘要:
Challenges remain in the generation of heterogeneous tissues and the repairing of interfacial tissue between soft and hard tissues. The development of tissue engineering scaffolds with gradients in composition, structure, mechanical and chemical properties is essential to modulate cellular behaviors in a graded way and potentially support the growth of functionally graded tissues. Integrated with the three-dimensional (3-D) nanofibrous skeletal structure of native extracellular matrix, electrospun fibers with gradients in amino groups were generated in the current study through an aminolysis process by using a microinfusion pump. Gelatin grafts were constructed to create fibrous scaffolds with gradients in hydroxyapatite (HA) contents, crystal size and mechanical properties through in situ mineralization. Plasmid DNA (pDNA) was included during the mineralization process, and gradations in pDNA loading contents were created on fibrous scaffolds on the basis of HA gradients. Obvious gradients in cell density, osteoblastic differentiation and collagen deposition were demonstrated along the long axis of fibrous mats after cell seeding. Gradients in the amount of pDNA released and the expression of target proteins were indicated on the fibrous mats, which offered a temporally and spatially controlled delivery of growth factors in scaffolds. The creation of gradient futures on 3-D fibrous scaffolds may provide physical, chemical and biological cues and result in efficient regeneration of tissues with spatial distributions of the cell proliferation, differentiation, and matrix secretion. (C) 2012 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.

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