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Polyethylenimine functionalized magnetic nanoparticles as a potential non-viral vector for gene delivery

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WOS被引频次:29
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成果类型:
期刊论文
作者:
Zhou, Yangbo;Tang, Zhaomin;Shi, Chunli;Shi, Shuai;Qian, Zhiyong;Zhou, Shaobing*
通讯作者:
Zhou, Shaobing
作者机构:
[Zhou, Yangbo; Zhou, Shaobing; Shi, Chunli] SW Jiaotong Univ, Sch Life Sci & Engn, Chengdu 610031, Sichuan, Peoples R China.
[Tang, Zhaomin; Zhou, Shaobing] SW Jiaotong Univ, Sch Mat Sci & Engn, Minist Educ, Key Lab Adv Technol Mat, Chengdu 610031, Sichuan, Peoples R China.
[Shi, Shuai; Qian, Zhiyong] Sichuan Univ, W China Hosp, W China Med Sch, State Key Lab Biotherapy, Chengdu 610041, Peoples R China.
通讯机构:
[Zhou, Shaobing] SW Jiaotong Univ, Sch Life Sci & Engn, Chengdu 610031, Sichuan, Peoples R China.
语种:
英文
期刊:
Journal of Materials Science: Materials in Medicine
ISSN:
0957-4530
年:
2012
卷:
23
期:
11
页码:
2697-2708
文献类别:
WOS:Article;EI:Journal article (JA)
所属学科:
ESI学科类别:材料科学;WOS学科类别:Engineering, Biomedical;Materials Science, Biomaterials
入藏号:
WOS:000310872400010;EI:20125215832398
基金类别:
National Basic Research Program of China (973 Program) [2012CB933602]; National Natural Science Foundation of China [30970723, 51173150]; Fundamental Research Funds for the Central Universities [SWJTU11ZT10]; Open Research Fund of the Key Laboratory of Biotherapy, West China Hospital, West China Medicine School, Sichuan University [SKLB200907]
机构署名:
本校为第一且通讯机构
院系归属:
材料科学与工程学院
生命科学与工程学院
摘要:
Polyethylenimine (PEI) functionalized magnetic nanoparticles were synthesized as a potential non-viral vector for gene delivery. The nanoparticles could provide the magnetic-targeting, and the cationic polymer PEI could condense DNA and avoid in vitro barriers. The magnetic nanoparticles were characterized by Fourier transform infrared spectroscopy, X-ray powder diffraction, dynamic light scattering measurements, transmission electron microscopy, vibrating sample magnetometer and atomic force microscopy. Agarose gel electrophoresis was used to asses DNA binding and perform a DNase I protection assay. The Alamar blue assay was used to evaluate negative effects on the metabolic activity of cells incubated with PEI modified magnetic nanoparticles and their complexes with DNA both in the presence or absence of an external magnetic field. Flow cytometry and fluorescent microscopy were also performed to investigate the transfection efficiency of the DNAloaded magnetic nanoparticles in A549 and B16-F10 tumor cells with (?M) or without (-M) the magnetic field. The in vitro transfection efficiency of magnetic nanoparticles was improved obviously in a permanent magnetic field. Therefore, the magnetic nanoparticles show considerable potential as nanocarriers for gene delivery. ©Springer Science+Business Media, LLC 2012.
参考文献:
Yao Z, Fenoglio S, Gao DC, Camiolo M, Stiles B, Lindsted T, Schlederer M, Johns C, Altorki N, Mittal V. TGF-β IL-6 axis mediates selective and adaptive mechanisms of resistance to molecular targeted therapy in lung cancer. PANS. 2010,107:15535–40.
Day ES, Morton JG, West JL. Nanoparticles for thermal cancer therapy. J Biomech Eng. 2009,131:074001.
Redd WH, Montgomery GH, DuHamel KN. Behavioral intervention for cancer treatment side effects. J Natl Cancer Inst. 2001,93:810.
Lungwitz U, Breunig M, Blunk T, Gopferich A. Polyethylenimine-based non-viral gene delivery systems. Eur J Pharm Biopharm. 2005,60:247–66.
Godbey W, Wu KK, Mikos AG. Poly (ethylenimine) and its role in gene delivery. J Controlled Release. 1999,60:149–60.

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